The interaction of triethyltin with a component of guinea-pig liver supernatant. Evidence for histidine in the binding sites.
نویسندگان
چکیده
A protein fraction was isolated from guinea-pig liver that binds triethyltin with an affinity of approx. 2x10(6)m(-1) at pH8.0. It was shown that the protein responsible for binding 70% of the triethyltin found in guinea-pig liver after injection of radioactively labelled triethyltin is at most a few per cent of the total liver protein. Evidence is presented from the kinetics of loss of binding and loss of certain amino acids on photo-oxidation with either Methylene Blue or Rose Bengal that each binding site consists of two histidine residues.
منابع مشابه
The interaction of triethyltin with components of animal tissues.
1. The distribution of triethyl[(113)Sn]tin chloride in the rat, guinea pig and hamster is not uniform, the highest concentrations being in rat blood and the liver of all three species. 2. Subcellular fractionation of rat liver, brain and kidney shows that triethyltin binds to all fractions to different extents. In the liver of the rat and guinea pig the supernatant fraction contains the larges...
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ورودعنوان ژورنال:
- The Biochemical journal
دوره 120 1 شماره
صفحات -
تاریخ انتشار 1970